An article published this year in “Biological Procedures Online” using one of our products, “APC Mouse anti-Human HLA-DR”, by our customers from NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Portugal, in the analysis of Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods. Congrats and Thanks.
Summay:
Dendritic cells (DCs), which can be
used as anti-cancer vaccines, are generally obtained in vitro from isolated CD14+ monocytes (MoDCs). This generates high cell numbers and
allows instructing DCs to guarantee effective antitumor responses. However, the
impact of the monocyte isolation step in the antitumor effectiveness of the
generated MoDCs is still unknown. Here, we compared the most used
immunomagnetic technologies for monocyte isolation: magnetic activated cell
sorting (MACS) from Miltenyi Biotec and EasySep from STEM CELL.
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