An article published this year in “Toxicology
In Vitro” using our “Apoptosis Detection Kit FITC”, by our customers from Torino, Italy, in the analysis of how Effects of the rare
elements lanthanum and cerium on the growth of colorectal and hepatic cancer
cell lines. Congrats and Thanks.
Summay:
Human HT-29 and HepG2 cell lines were employed to test
the effects of increasing concentrations of two rare earth elements (REEs),
namely cerium (Ce) and lanthanum (La), alone or in combination. Effects on cell
proliferation were measured using MTT assay, luciferase-based assays and proliferating
cell nuclear antigen expression, while cell mortality and type of cell death
was determined by Annexin V-FTC test using flow cytometry. Modulation of 84
genes involved in oxidative stress pathways was also studied using RT-PCR based
arrays. Major alterations in selected genes compared to basal expression levels
of respective control groups were found in the cells exposed to 600 µM Ce for
48 h. In HepG2 cells, 51 out of 84 genes were significantly up- or
down-regulated, while in HT-29 cells only 16 genes were significantly up- or
down-regulated. Dosage of REEs seems to be the pivotal factor for switching the
biological effects from down- to up-regulation of cell growth; thus, low
concentrations promoted cell survival and proliferation, but when concentrations
increased, REEs exerted anti-proliferative and cytostatic/cytotoxic effects.
The molecular mechanisms underlying these effects are still not well-defined
and further analysis of the mechanisms that result in inhibition or induction
of cell proliferation are crucially important.
Reference:
Product link:
Apoptosis Detection Kit FITC
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